human gbm cell lines ln229  (ATCC)

Journal: Nature Communications

Article Title: Iontronic click-to-release enables electrically controlled delivery of drugs and biomolecules beyond charge and size limitations

doi: 10.1038/s41467-026-70985-0

Figure Lengend Snippet: a Schematic of the synthesis and immobilization strategy for biotinylated CA4-prodrug ( 6 ), synthesized via sequential decoration of cTCO-bis-NHS ( 5 ). The biotin handle allows immobilization onto streptavidin-coated magnetic beads, forming a localized, iontronically activatable prodrug reservoir. IPs were positioned above the beads in a transwell insert containing U-87 cells in the lower chamber. Grey pills indicate inactive (bound) drug, blue (colored) pills indicate active drug. b 3D-printed platform designed to precisely position iontronic devices in standard 96-well transwell plates. c Cell viability of U-87 cells after operating iontronic devices delivering 1 for 4 h at +20 nA (Active, n = 8) and at −20 nA (Reverse, n = 7) into transwells holding CA4-immobilized beads from ( a ). Points represent individual biological replicates; bars indicate mean ± SD. GC growth control (untreated, n = 32), “No Beads” = operation of iontronic devices delivering 1 at +20 nA, no beads in transwell present ( n = 6), “No Tz 1 ” = CA4-immobilized beads in transwell present with iontronic delivery of K + (0.1 M KCl) instead of Tz 1 ( n = 8). Statistical analysis was done via a Brown–Forsythe and Welch ANOVA tests (one-way) and Dunnett’s T3 multiple comparisons test. α = 0.05. p -values: GC vs. No Beads (ns) = 0.2377; GC vs. No Tz 1 (ns) = 0.3827; GC vs. Reverse, 4 h (ns) = 0.5739, GC vs. Active, 4 h (****) = <0.0001. Certain graphical elements in ( a ) were created in BioRender. Hecko, S. (2026) https://www.BioRender.com/z8yr1eu .

Article Snippet: The human GBM cell line U-87 (provided by the MUG Cell Bank, catalogue no. 300367, CLS) was cultured at 37 °C and 5% CO 2 in Eagle’s minimum essential medium (E-MEM) containing 10% fetal bovine serum (FBS), 4.5 g/L glucose, 2 mM L -glutamine, and 1% MEM Non-Essential Amino Acids.

Techniques: Synthesized, Magnetic Beads, Control

Journal: Nature Communications

Article Title: Iontronic click-to-release enables electrically controlled delivery of drugs and biomolecules beyond charge and size limitations

doi: 10.1038/s41467-026-70985-0

Figure Lengend Snippet: a Schematic overview of the bioorthogonal click-to-release (C2R) cascade of CA4-prodrug: Aminoethyl tetrazine (Tz 1 ) triggers the release of the cytotoxic agent combretastatin A-4 ( CA4 ) from sulfo-cTCO-DMEDA-CA4 ( 4 ) via a tetrazine-triggered elimination from TCO caged payload and self-immolation. b Dose-dependent cell viability of human glioblastoma cell line U-87 after 72 h incubation with the key components of the C2R reaction. Data represent mean ± SD of n = 3 independent biological replicates. Curves were fit using four-parameter logistic regression. Prodrug 4 (0.01–1000 nM, grey curve); parent CA4 (0.01–1000 nM, blue curve); released CA4 (0.01–1000 nM 4 + 5 µM 1, dashed blue curve); Tz 1 (5 µM, purple data point). c Cell viability of U-87 cells after operating iontronic devices delivering 1 for 1 h at +20 nA (Active, n = 9) and at −20 nA (Reverse, n = 11). Points represent individual biological replicates; bars indicate mean ± SD. GC growth control (untreated, n = 12). Statistical analysis was done via a Brown–Forsythe and Welch ANOVA tests (one-way) and Dunnett’s T3 multiple comparisons test. α = 0.05. p -values: GC vs. Reverse, 1 h (ns) = 0.5339; GC vs. Active, 1 h (****) = <0.0001. Certain graphical elements in ( a ) were created in BioRender. Hecko, S. (2026) https://www.BioRender.com/z8yr1eu .

Article Snippet: The human GBM cell line U-87 (provided by the MUG Cell Bank, catalogue no. 300367, CLS) was cultured at 37 °C and 5% CO 2 in Eagle’s minimum essential medium (E-MEM) containing 10% fetal bovine serum (FBS), 4.5 g/L glucose, 2 mM L -glutamine, and 1% MEM Non-Essential Amino Acids.

Techniques: Incubation, Control

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: IDH1 and IDH1 R132H expression in U-87 MG and U-87 MG IDH1 R132H . Quantification of IDH1 ( A ) and IDH1 R132H ( B ) normalized to GAPDH. Data are shown via interleaved bars plotting mean ± SD of n = 3 independent experiments; **** p -value < 0.0001 vs. IDH1.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Cell growth evaluation after B-SRCi exposure. Evaluation of cell growth 24 h after B-SRCi treatment exposure at 0.1 µM, 1 µM, 10 µM, and 100 µM of ( A ) in U-87 MG and ( B ) U-87 MG IDH1 R132H lines; data were shown via column mean, error bars and mean connected ± SD of n = 3 independent experiments; * p -value < 0.05; ** p -value < 0.01; **** p -value < 0.0001 vs. 0 μM.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques:

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Boron uptake measurement in U-87 MG and U-87 MG IDH1 R132H cell lines. Grayscale image of the sample (intensity 0–255) ( A , E ); 2D map of the 10 B concentration reconstructed using the calibration curve, expressed in ppm (color bar on the right) ( B , F ); histogram of the grayscale-level distribution (A.U.) within the analyzed area (the orange crosses indicate the main maxima (peaks) of the distribution, subsequently used to estimate the 10 B concentration) ( C , G ); histogram of the distribution of 10 B concentration values (ppm) corresponding to the pixels of the 2D map ( D , H ). The crosses mark the local maxima identified in the grayscale histogram. Each marked peak is used as a seed for a Gaussian fit performed in a neighborhood around the maximum, which provides a robust estimate of the peak position and amplitude for subsequent conversion to boron concentration.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Concentration Assay

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: pSRC (Tyr416) expression in U-87 MG and U-87 MG IDH1 R132H . ( A ) Quantification of pSRC protein normalized to GAPDH and ( B ) representative blots. Data are shown as interleaved bars plotting mean ± SD of n = 3 independent experiments; * p -value < 0.05 and ** p -value < 0.01.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Gene expression evaluation after B-SRCi exposure. Evaluation of gene expressions in U-87 MG and in U-87 MG IDH1 R132H lines exposed to 10 µM B-SRCi treatment 24 h; evaluation of ( A , B ) SRC, ( C , D ) BCL-2, ( E , F ) BAX, ( G , H ) CASP-3, and ( I , J ) CASPASE-8 in both cell lines; data are shown as interleaved bars, mean ± SD of n = 3 independent experiments; * p -value < 0.05; *** p -value < 0.001; **** p -value < 0.0001 vs. vehicle (0 μM).

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Gene Expression

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Immunofluorescence evaluation in U-87 MG and in U-87 MG IDH1 R132H lines exposed to 10 µM B-SRCi treatment 24 h; Ki-67 nuclear Corrected Total Cellular Fluorescence in Arbitrary Units (CTCF a.u.) of U-87 MG ( A , B ) and U-87 MG IDH1 R132H ( C , D ); CASP-3 cytoplasmic Corrected Total Cellular Fluorescence in Arbitrary Units (CTCF a.u.) of U-87 MG ( E , F ) and U-87 MG IDH1 R132H ( G , H ) data are shown as interleaved bars, mean ± SD of n = 3 independent experiments; ** p -value < 0.01; *** p -value < 0.001; **** p -value < 0.0001 vs. vehicle (0 μM). Nuclei are stained with DAPI (blue). Caspase and Ki67-positive cells are shown in green (Scale bar 20 μm).

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Immunofluorescence, Fluorescence, Staining

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Rate of cell growth of proton-irradiated U-87MG cell line in combination with B-SRCi. Evaluation of R.C.G. in U-87 MG cell line exposed to 10 µM B-SRCi combined with protons at doses of 1, 2, 4, and 5 Gy after 24 h ( A ), 48 h ( B ), and 72 h ( C ); data are plotted as points and connecting lines, with error bars showing the mean ± SD of n = 3 independent experiments; ** p -value < 0.01 and **** p -value < 0.0001 for Vehicle + radiation dose vs. 0 Gy Vehicle (sham-irradiated); # p -value < 0.05, ## p -value < 0.01, ### p -value < 0.001 and #### p -value < 0.0001 for B-SRCi + radiation dose vs. 0 Gy vehicle (sham-irradiated); R.C.G rate of cell growth.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Irradiation

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Rate of cell growth of the proton-irradiated U-87 MG IDH1 R132H cell line in combination with B-SRCi. Evaluation of R.C.G. in U-87 MG IDH1 R132H cell lines exposed to 10 µM B-SRCi combined with protons at doses of 1, 2, 4, and 5 Gy after 24 h ( A ), 48 h ( B ), and 72 h ( C ); data are plotted as points and connecting lines, with error bars showing the mean ± SD of n = 3 independent experiments; ** p -value < 0.01 and *** p -value < 0.001 for Vehicle + radiation dose vs. 0 Gy vehicle (sham-irradiated); ### p -value < 0.001 and #### p -value < 0.0001 for B-SRCi + radiation dose vs. 0 Gy vehicle (sham-irradiated); R.C.G rate of cell growth.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Irradiation

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Rate of cell growth of the X-ray-irradiated U87-MG cell line in combination with B-SRCi. Evaluation of R.C.G. in U-87 MG cell lines exposed to 10 µM B-SRCi combined with X-ray at 1, 2, 4, and 5 Gy after 24 h ( A ), 48 h ( B ), and 72 h ( C ); data are plotted as points and connecting lines, with error bars showing the mean ± SD of n = 3 independent experiments; * p -value < 0.05 for Vehicle + radiation dose vs. 0 Gy vehicle (sham-irradiated); ### p -value < 0.001 for B-SRCi + radiation dose vs. 0 Gy vehicle (sham-irradiated); R.C.G rate of cell growth.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Irradiation

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: Rate of cell growth of the X-ray-irradiated U-87 MG IDH1 R132H cell line in combination with B-SRCi. Evaluation of R.C.G. in U-87 MG IDH1 R132H cell lines exposed to 10 µM B-SRCi combined with X-ray at 1, 2, 4, and 5 Gy after 24 h ( A ), 48 h ( B ), and 72 h ( C ); data are plotted as points and connecting lines, with error bars showing the mean ± SD of n = 3 independent experiments; ++++ p -value < 0.0001, +++ p -value < 0.001 and ++ p -value < 0.01 for Vehicle vs. B-SRCi at the same dose; * p -value < 0.05, ** p -value < 0.01 and **** p -value < 0.0001 for Vehicle + radiation dose vs. 0 Gy vehicle (sham-irradiated); # p -value < 0.05, ### p -value < 0.001 and #### p -value < 0.0001 for B-SRCi + radiation dose vs. 0 Gy vehicle (sham-irradiated); R.C.G rate of cell growth.

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Irradiation

Journal: Pharmaceuticals

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines

doi: 10.3390/ph19030392

Figure Lengend Snippet: DNA damage by γH2A.X evaluation 30 min after X-ray and proton irradiation of U87-MG and the cell line in combination with B-SRCi. Evaluation of mean number of γH2A.X per nucleus in U-87 MG cell lines ( A ) and representative images ( B ) 30 min after irradiation with X-rays and protons at 2 Gy with and without 10 µM B-SRCi treatment; data are shown as a scatter plot, mean ± SD of n = 3 independent experiments; **** p -value < 0.0001 and *** p -value < 0.001, vs. 0 Gy vehicle (sham-irradiated); #### p -value < 0.0001 and # p -value < 0.05, vs. B-SRCi; +++ p -value < 0.001. Nuclei are stained with DAPI (blue). γH2AX foci are detected in red (anti-rabbit IgG–Atto 594). Scale bar 20 μm (5 μm for ROI).

Article Snippet: U-87 MG human GBM cell lines were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA).

Techniques: Irradiation, Staining